P-102: The Effect of Strontium on Growth and Development of Two-Cell Arrested NMRI Mouse Embryos

Background: Decreasing the growth and developmental rate of embryo and arresting in certain step of development like two cell block could be the reason of infertility in some couples referring to fertility and infertility center. The aim of this study is the effect of strontium on growth and development of two-cell arrested NMRI mouse embryo. Materials and Methods: The female mice were coupled with males following superovulation and positive vaginal plaque mice were killed 48 hour after HCG injection by cervical dislocation. Subsequently two-cell embryos were collected in Roswell Park Memorial Institute (RPMI) and divided and cultured (in M16 medium) in three groups. The 1st group were washed and incubated without any exposure. The 2nd and 3rd groups were exposed to 4°C for 24 hours in order to delay and arrest for cleavage rate. The 2nd group was incubated immediately, while the 3rd group was exposed to 10 mM strontium for 5 minutes. Results: The data analysis by one-way Anova shows that the rate of degenerated embryos have significantly differences (p=0.006) between groups by low temperature (4°C) exposure. Our results shows that the mean percent of blastocyst formation (33.4%) and cleavage rate (45.2%) in 2nd group were decreased related to 1st group (%77.7 and 90.9% respectively). With effect of strontium in 3rd group, the cleavage rate hasn’t any significant differences with 2nd group (45.2% related to 45.5% respectively), while the blastocyst formation rate were significantly (p=0.019) increased (61%) related to 2nd group. Conclusion: Developmental rate of mouse arrested two-cell embryos were significantly increased by effect of strontium related to control group.